MEASURE FLUORESCENCE in a sentence | Sentences WordTool (2024)

It allows to measure fluorescence levels in closely associated sperm tails, through an exact distinction between positive and background signals, and provides background - corrected pixel intensity values that can directly be used for data analysis.

Another innovation of FLEX is its ability to measure fluorescence associated with two different steps in the photosynthetic system — one in the red and one in the infrared.

Color is used to indicate the measured fluorescence emission position of each single molecule.

For endpoint analysis, the core contains two automated plate readers, an MSD Sector Imager that measures electrochemiluminescence for high - sensitivity and multiplex assays, and a BioTek Synergy H4 Hybrid Plate Reader that measures both fluorescence and colorimetric readouts.

The team measured the fluorescence from large areas of crops in the Midwestern Corn Belt and the Indo - Gangetic Plain.

Cox obtained a biology degree at the University of Wolverhampton in the United Kingdom and went on to do a biochemical engineering Ph.D. at the University of Birmingham where he measured the growth of filamentous fungi in solid pastes using image analysis and fluorescence microscopy.

Energy transfer between the subunits, a measure of cytosolic cAMP concentration -LRB-[cAMP]-RRB-, and compartmentation of the dissociated subunits were monitored by confocal fluorescence microscopy.

But the fluorescence map, an unexpected secondary capability, provides a more direct measure of carbon fluxes: the amount mopped up by plants during photosynthesis or released during respiration.

And the satellite will also spy on plants» carbon uptake by measuring the weak fluorescence that is produced by chlorophyll during photosynthesis.

Measuring the variations in the intensity of the resulting fluorescence provides a «dynamic readout of cellular metabolism» which is a sensitive biomarker of drug response.

When they inserted a fluorescent reporter protein in this virus - like shell, their test became capable of high - speed screening to see which drugs blocked the entry of Ebola - like viral particles into cells as measured by fluorescence.

The researchers began by creating large membrane models with embedded and dyed channel proteins; they then put them on a glass surface and measured them using a single - molecule measuring method known as fluorescence correlation spectroscopy.

To study the plume, scientists have been lowering instruments from research ships to take fluorescence measurements of the water at different depths and to measure the amount of dissolved oxygen, among other things.

The Illinois team devised a way to measure how diffusion slows down when a protein unfolds using a fluorescence microscope, then used three - dimensional diffusion models to connect the protein's unfolding to its motion.

However, by combining the capabilities of the Surface Forces Apparatus (SFA)-- a device that can measure the tiny forces generated by the interaction of two surfaces at the sub-nano scale — and simultaneous imaging using a fluorescence microscope, the researchers were able to see in real time how the cell membranes rearrange in order to connect and open a fusion conduit between them.

Chlorophyll fluorescence, antioxidant capacity, phenolic concentration, and flavonoid concentration were also measured in the study.

To prove their concept of this multiplex spectral microgel analysis within a microfluidic flow, the team used «different barcodes corresponding to different emissions at specific wavelengths and the fluorescence intensity of known microRNA concentration,» which was measured for calibrations of the specific microRNA being explored.

Since both sensors were previously calibrated, the measured changes in fluorescence corresponded to the application of a certain amount of force.

When a laser is passed over the green protein, it changes to red as a way of tagging when it has been been translated, and new proteins synthesized at that hotspot would be green, which is visible by the appearance of yellow fluorescence (green + red, as measured by light on the visible spectrum).

OCO - 2 will also closely monitor the carbon uptake of plants by measuring the weak fluorescence that is produced during photosynthesis as plants» chlorophyll pigments absorb light to capture energy and subsequently re-emit photons at longer wavelengths.

Researchers now use X-ray fluorescence spectrometry to measure air pollution metal content, ion chromatography to identify other chemicals and other tactics to assess organic and elemental carbon levels.

By developing a new fluorescence microscopy - based technique, the researchers were able to measure how long it takes proteins to move over distances ranging from 0.2 to 3 micrometres in living cells.

GFP fluorescence was measured by flow cytometry and normalized to control cells treated with DMSO.

Cell fluorescence was measured with the FACSCalibur or LSRII (BD Biosciences).

«Time Resolved Fluorescence Resonance Energy Transfer» - a sophisticated technique for measuring interactions between antibodies.

The quality of the data set is enhanced by the possibility to combine the information from two different detectors: the particle density at the ground is measured with a surface array of water Cherenkov detectors whereas the longitudinal development of the air showers is observed with fluorescence telescopes during dark clear nights.

We, therefore, treated macrophages of six AD patients and 3 controls by curcuminoids in vitro and measured Aβ uptake using fluorescence and confocal microscopy.

We used a fluorescence - based microplate respirometer to measure the oxygen consumption of coral larvae from both species, and also assessed the effects of these stressors on P. astreoides larval settlement and mortality.

Responses were correlated to calcium release in cultured rat uterine smooth muscle cells measured by fluorescence microscopy.

In FRAP assays (FRAP: Fluorescence Recovery After Photobleaching), the time that fluorescent molecules need to replenish a bleached - out area is measured,...

Ligand - mediated receptor assembly was measured by photon transfer from the photon donor to the fluorophore resulting in fluorescence emission.

One cycle of denaturing step (10 minutes at 95 °C) was applied, followed by 35 cycles of amplification (15 seconds at 95 °C and 1 minute at 58 °C), with fluorescence measured during the extension.

Briefly, 150 islets were incubated in 10 µM dichlorofluorescein diacetate at 37 °C for 30 min and the resulting fluorescence was measured with excitation of 495 nm and emission of 538 nm.

We use near - surface cameras and field - based spectroradiometers and fluorescence spectroscopy and link ground - based measures of phenology with imagery data obtained from satellites.

After washing, Luciferase substrate was added for 30 minutes and fluorescence was measured on an Envision reader.

Peptides were added to the culture medium, and fluorescence changes from GCaMP6s were measured by live imaging.

To directly measure the level of coordination in the expression of genes, we used highly sensitive fluorescence in situ hybridization (FISH) to count individual mRNAs of functionally related and unrelated genes within single Saccharomyces cerevisiae cells.

Response: We originally used the DAB method to determine SNpc cell counts, and once we determined that there were no differences we sought to measure astrogliosis, which required fluorescence detection of both TH and GFAP.

For densitometry analysis, the fluorescence intensity of individual cells stained for p - Smad1 / 5 was measured using ImageJ analysis software.

Calcein - AM and Hoechst were added at the end of the treatment for 10 minutes and the developed fluorescence was measured in assay buffer in a Victor3 plate reader (Hoechst at excitation and emission wavelengths of 355 nm and 465 nm, respectively and calcein at excitation and emission wavelengths of 485 nm and 535 nm, respectively).

The viability of each individual cell was monitored by measuring the level of fluorescence of specific vital dyes that leak out from the damaged cell (PNB - induced cell damage was shown to have a disruptive mechanical nature [40,45,47]-RRB-.

Several methods to measure FRET by fluorescence microscopy can be used, including fluorescence lifetime imaging, spectral imaging, donor fluorescence dequenching upon acceptor photobleaching.

Fluorescence - based assays Fluorescent markers such as EGFP, YFP, mCherry and mTomato or fluorescent biosensors can be used to measure a variety of real - time cell - based activities, including, intracellular transport, protein signaling, receptor desensitization, migration, division, apoptosis, metabolism, differentiation, chemotaxis, transcription and translation.

Fluorescence intensities were measured at an excitation wavelength of 340 or 380 nm and an emission wavelength of 510 nm, with a fluorescence spectrometer (Hitachi F - 2500) during stimulation as indicateFluorescence intensities were measured at an excitation wavelength of 340 or 380 nm and an emission wavelength of 510 nm, with a fluorescence spectrometer (Hitachi F - 2500) during stimulation as indicatefluorescence spectrometer (Hitachi F - 2500) during stimulation as indicated in Fig. 5.

Conducted research, handled purchasing and developed x-ray fluorescence system to measure thin film compositions and thickness on wafers.